FIG 8.

Expressing mapA under the control of the lapA promoter does not rescue loss of LapA function. (A and B) Quantification of biofilm formed by WT P. fluorescens Pf0-1 and ΔlapA, Pswap, and ΔlapA Pswap mutants after 6 h of growth in KA medium (A) or 16 h of growth in KA medium (B). (C) Cell surface expression of MapA in the WT and Pswap strains. The cell surface expression of LapA is shown as an additional control. Shown is one representative experiment of three biological replicates. (D) Quantification of the cell surface expression of HA-MapA in the WT and Pswap strains and of HA-LapA in the WT strain, as indicated. Quantification was performed as described in the legend for Fig. 3. For statistical tests, all hypothesis testing was conducted using a two-tailed t test. *, P < 0.05; ns, P > 0.05.