TABLE 3.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| Saccharomyces cerevisiae InvSc1 | MATa/MAT leu2/leu2 trp1-289/trp1-289 ura3-52/ura3-52 his3D1/his3D1 | Invitrogen |
| Escherichia coli S17-1 (λpir) | Tpr Smr recA thi pro hsdR-M+RP4::2-Tc::Mu::Km Tn7 λpir | 78 |
| Pseudomonas fluorescens Pf0-1 | ||
| SMC 1358 | WT Pf0-1 (both LapA and MapA untagged) | |
| SMC 4798 | LapA-HA; HA tag inserted after residue 4093 | 88 |
| SMC 8581 | MapA-HA | This study |
| SMC 7905 | LapA-HA MapA-HA | This study |
| SMC 8586 | ΔlapA MapA-HA | This study |
| SMC 7977 | ΔmapA LapA-HA | This study |
| SMC 8570 | ΔlapA ΔmapA | This study |
| SMC 8582 | ΔlapD LapA-HA MapA-HA | This study |
| SMC 8584 | ΔlapG LapA-HA MapA-HA | This study |
| SMC 8594 | ΔlapAG MapA-HA | This study |
| SMC 8569 | ΔlapAG ΔmapA | This study |
| SMC 8588 | ΔmapE LapA-HA MapA-HA | This study |
| SMC 8589 | ΔlapA ΔmapE MapA-HA | This study |
| SMC 8591 | ΔlapE ΔmapE LapA-HA | This study |
| SMC 7954 | ΔlapE LapA-HA | 29 |
| SMC 8636 | ΔlapE ΔmapA LapA-HA | This study |
| SMC 8638 | PmapA::PlapA (also known as Pswap) LapA-HA MapA-HA | This study |
| SMC 8639 | ΔlapA PmapA::PlapA MapA-HA | This study |
| SMC 8642 | mTn7 PlapA-GFP PmapA-mCherry LapA-HA MapA-HA (also known as Pfluor) | This study |
| Plasmids | ||
| SMC 7221 | mTn7/pMQ56 | 79 |
| SMC 1852 | pUX-BF13 tnsA tnsC tnsD tnsE mob ori R6K bla MCS | 89 |
| SMC 2765 | pMQ30 | 79 |
| SMC 7975 | pMQ30 ΔmapA | This study |
| SMC 7907 | pMQ30 MapA-HA | This study |
| SMC 8703 | pMQ30 ΔmapE | This study |
| SMC 8637 | pMQ30 PmapA::PmapA | This study |
| SMC 8635 | pMQ56 mTn7 plapA-GFP, pmapA-mCherry | This study |