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Clp proteins are needed to avoid manganese toxicity during H2O2 stress. Anaerobic cultures were diluted into aerobic medium at time zero, and 4 μM manganese was added. The addition of branched-chain amino acids (ILV) suppressed the manganese-dependent growth defect. Growth inhibition by manganese was not observed in Hpx+ or Clp+ strains (Fig. S10). Strain: ASE125.
