FIG 5.
Replication fitness does not predict transmission fitness. Multivirus infection assays in penile tissue were performed, and the average replication of each Envchronic and each Envacute virus in tissue (a) and in MC+PM1 cocultures (b) was determined by sequencing. (c) Each of the Envacute viruses was competed against the Envchronic controls in PHA- and IL-2-activated PBMCs to assess the pathogenic fitness (21). The average pathogenic fitness versus the three Envchronic viruses is shown. (d) The percent replication of Envacute viruses in competitions in tissue and in MC + PM1 cocultures was plotted against the average percent replication in PBMC competitions. ND, not done.