FIG 6.
Binding profile of Envacute and Envchronic viruses to C-type lectins. Binding to DC-SIGN and langerin was determined using an acoustic biosensor. Binding affinity (dissociation constant [Kd], in nanomolar units) was determined by binding the lectins of interest to the chip and allowing viruses to flow over the surface. Average replication in tissue of Envacute (red squares) and Envchronic (green squares) viruses was correlated with the binding affinity to DC-SIGN (a), langerin (b), and PHA (c). Average transmission fitness in MC+PM1 cocultures of Envacute (red squares) and Envchronic (green squares) viruses was plotted against the binding affinity to DC-SIGN (d), langerin (e), and PHA (f). PHA was tested as the nonspecific control. Each symbol represents one virus. Statistical analysis was performed using a Spearman rank correlation analysis.