FIG 8.

Separation of HBeAg and PreC proteins from HBV virion particles in patient sera by CsCl density gradient fractionation. Sera from patients infected with the indicated HBV genotype (A, B, C, or D) were fractionated by CsCl density gradient ultracentrifugation. Fractions were resolved by regular SDS-PAGE, followed by immunoblot analysis using MAb 1A11, T2221, or 366-2. Fractions from patient 3 (genotype A or gtA) (A) were resolved by SDS-PAGE in the high-resolution gel, and fractions from patient 4 (gtB) (B), patient 10 (gtC) (C), and patient 12 (gtD) (D) were resolved by SDS-PAGE in a regular gel. V, the virion peak of each gradient as determined by native agarose gel electrophoresis shown in Fig. 6. The direction of centrifugation (top to bottom) is indicated.