FIG 4.

VC2 treatment promotes infiltration of T cells into tumors. (A) PBS- or VC2-treated B16F10n-1 tumors were removed from mice 1 day after the third treatment and processed for immunohistochemistry. Tissues were stained with anti-mouse CD3 (brown) antibody and a counterstain. (B) CD4⁺ (brown) and CD8⁺ (red) double staining of the same tumors. Scale bar, 50 μm. Immunostaining was scored based on the cumulative number of positive cells in five high-magnification (40×) microscopic fields. Representative images from 5 mice per group are shown. *, P < 0.05; **, P < 0.005.