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. 2021 Jan 13;95(3):e02114-20. doi: 10.1128/JVI.02114-20

FIG 7.

FIG 7

BAF depletion partially rescues ΔB1 and ΔB1mutB12 virus in VRK1-depleted CV1 cells but does not enhance replication in HAP1-VRK1KO cells. (A) Immunoblot using anti-BAF and anti-VRK1 in CV1 cells transduced to express control shCTRL or shBAF and then treated with siVRK1 or a control siRNA. (B) CV1 cells depleted as shown in panel A were infected with WT, ΔB1, or ΔB1mutB12 virus at an MOI of 3 and harvested at 24 hpi for viral yield determination. (C) Protein immunoblotting using anti-tubulin and anti-BAF in shBAF-depleted HAP1 and HAP1-VRK1KO cells. (D) Viral yield measured for WT, ΔB1, and ΔB1mutB12 viruses at 24 hpi in transduced HAP1 and VRK1KO cell lines infected at an MOI of 3. (E) GAPDH-normalized vaccinia DNA accumulation measured for WT, ΔB1, and ΔB1mutB12 viruses at 24 hpi in transduced HAP1 and VRK1KO cell lines infected at an MOI of 3. For all immunoblots, open triangles indicate phosphorylated BAF, and closed triangles indicate unphosphorylated BAF. ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.