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Binding of unlabeled (white) and labeled (gray) GII.4 VLPs to synthetic carbohydrates. Streptavidin plates were coated overnight at 4°C with 10 μg/ml PAA-conjugated HBGAs, and 100 ng of VLPs was incubated overnight at 4°C. Bound labeled and unlabeled VLPs were detected with an anti-FITC and an anti-GII.4 antibody, respectively. Each reaction was done in duplicates, and the error bars represent the standard deviations.
