TABLE 1.
Differential gene expression in SAMHD1 KO THP-1 cells compared to control cells
| Gene symbol | Entrez gene name | mRNA fold change | Expected activationa |
|---|---|---|---|
| KRAS | KRAS proto-oncogene, GTPase | −5.42 | Up |
| HDAC2 | Histone deacetylase 2 | −3.90 | Down |
| TAB2 | TGF-beta activated kinase 1/MAP3K7 binding protein 2 | −3.37 | Up |
| MAP3K7b | Mitogen-activated protein kinase kinase kinase 7 | −3.25 | Up |
| IGF2R | Insulin-like growth factor 2 receptor | −2.95 | Up |
| PIK3R1 | Phosphoinositide-3-kinase regulatory subunit 1 | 2.23 | Up |
| PRKACB | Protein kinase cAMP-activated catalytic subunit beta | 2.12 | Up |
| PIK3C3 | Phosphatidylinositol 3-kinase catalytic subunit type 3 | 2.23 | Up |
| FCER1G | Fc fragment of IgE receptor Ig | 2.51 | Up |
| IL18 | Interleukin 18 | 3.49 | Up |
| CD40 | CD40 molecule | 3.63 | Up |
| TLR6 | Toll-like receptor 6 | 3.68 | Up |
| AKT3 | AKT serine/threonine kinase 3 | 10.71 | Up |
a
The expected activation of genes whose mRNA expression levels were significantly altered in THP-1 SAMHD1 KO cells relative to control cells (fold change > 2; P ≤ 1 × 10−4) was predicted through ingenuity pathway analysis (IPA) of the microarray data. RNA extracted from triplicate THP-1 control and SAMHD1 KO cells was analyzed by Affymetrix Clariom D microarray (29).
b
MAP3K7 is also known as transforming growth factor β-activated kinase 1 (TAK1).