FIG 7.

Founder effect of D614-variants in Spain and selective advantage of D614G substitution. (A) D614/G614 prevalence over time in Spain according to the data available on 8 July 2020. Increased infectivity of D614G pseudoviruses. VeroE6, Caco-2, and 293T cells expressing human ACE2 were infected with equal amounts (10 ng of Gag p24) of D614 or G614 pseudoviruses. Viral infectivity was determined by measuring luciferase activity in the cell lysates 48 h postinfection and is expressed as percent infectivity relative to that of D614 virus. Results are means and standard deviations for an experiment performed in triplicate. Results of a representative experiment out of three independent assays is shown. ****, P < 0.0001; ***, P < 0.001; **, P < 0.01 (unpaired two-tailed Student's t test).