TABLE 2.
Biophysical properties of AMC009 and AMC011 SOSIP trimers
| Parameter | Value by Env trimer and SOSIP versionc
|
|||
|---|---|---|---|---|
| AMC009 |
AMC011 |
|||
| 4.2 | 5.2 | 4.2 | 5.2 | |
| Productiona (yield [mg/liter]) | 1.6b | 1.52b | 4.75 | 2.1 |
| Morphology by NS-EM (% native-like trimers) | 100b | 100 | ND | 100 |
| Thermostability by DSC (two-state model [Tm in °C]) | 68 | 70 | 63 | 67 |
| Glycan composition by HILIC-UPLC (%) | ||||
| Man5–9 | 72 | 77*b | 72 | 78* |
| Man9 | 34 | 32*b | 37 | 32* |
a
Results were obtained from 293F cell-expressed and PGT145-purified SOSIP trimers.
b
Results were obtained with D7324-tagged proteins.
c
ND, not determined; *, quantified without Endo H digestion.