FIG 7.

Production of interferons, IP-10, and IL-6 in RSV mutant-infected HBE cultures. The apical andbasolateral fluid from the infected HBE cultures described in the legend to Fig. 6 were sampled as described in the legend to Fig. 5 at the time that each mutant-infected culture reached its maximum GFP expression (indicated below each pair of bars). IL-6 (A), IP-10 (B), IFN-λ1 (C), IFN-λ2/3 (D), and IFN-β (E) levels were assayed using the Legendplex human antivirus response panel (Biolegend) bead assay. Basolateral values were multiplied by 5 to account for the dilution in 5 times more liquid. The data from rgRSV mutants were compared to those from rgRSV. Statistical analysis is indicated. Data with no significant difference (P > 0.05) are not labeled in the figure panels. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.