FIG 1.

Self-limiting replication of GAstV-GD in LMH cells. (A) LMH cells infected with GAstV-GD (a) or not infected (b) were reacted with the mouse sera against ORF2 of GAstV-GD by indirect immunofluorescence assay. (B) The growth curve of GAstV-GD in LMH cells. LMH cells were infected with GAstV-GD at a multiplicity of infection (MOI) of 0.1 and cultured with or without trypsin-tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK). At the indicated number of days postinfection, the titer of the supernatant of the infected cells was determined by 50% tissue culture infective dose (TCID₅₀). (C) Cell activity assay of GAstV in LMH cells. At different time points postinfection, the activity of the infected cells was determined by a CCK-8 approach comparing with activity of the wild-type LMH cells. All experiments were done in triplicate and repeated twice.