FIG 2.

GAstV-GD activated innate immunity in vitro and in vivo. Transcriptional levels of TLR3, TRIF, IKKα, IFN-β, NF-κB, Mx1, OASL and TNF-α associated with innate immunity in GAstV-GD infected LMH cells and control cells were analyzed at 24 hpi (A), 48 hpi (B), 72 hpi (C) and 96 hpi (D); (E) Viral titers in the liver and kidney from the GAstV-GD infected goslings (n = 5) and control goslings (n = 5); (F) Transcriptional level of OASL in the liver and kidney from the GAstV-GD infected goslings (n = 5) and control goslings (n = 5). All experiments were done in triplicates and repeated twice.