FIG 5.

OASL could efficiently inhibit the replication of GAstV-GD. Growth curves of GAstV-GD at 0.01 MOI in the wild-type LMH cells and the LMH_KD-OASL cells which were transfected with pcDNA 3.1_OASL and pcDNA 3.1 before infection. At the indicated number of days postinfection, titer of the supernatant of the infected cells was determined by TCID₅₀ (A), and the infected cells were analyzed at 5 dpi by Western blotting (B). Lanes 1 and 2 show the wild-type LMH cells transfected with pcDNA 3.1 and pcDNA 3.1_OASL, respectively; lanes 3 and 4 show the LMH_KD-OASL cells transfected with pcDNA 3.1 and pcDNA 3.1_OASL, respectively. All experiments were done in triplicate and repeated twice.