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. 2020 Nov 23;94(24):e01767-20. doi: 10.1128/JVI.01767-20

TABLE 1.

Primer list for truncation of GAstV ORF2

Primer name Direction Sequence (5′–3′)
pc Forward GAATTCTGCAGATATCCAGCACAGTG
pc Reverse GCTCGGTACCAAGCTTAAGTTTAAACG
pcORF2-flag Forward AGCTTGGTACCGAGCATGGATTACAAGGATGACGACGATAAGATGGCAGACAGGGCGGTG
pcORF2-flag Reverse ATATCTGCAGAATTCTTATCACTCATGTCCGCCCTTCTC
pcORF2-S-flag Reverse ATATCTGCAGAATTCTTATGGCTTTGGACCATAGTTCGAG
pcORF2-S+P1-flag Reverse ATATCTGCAGAATTCTTACATTGGTGCCACTGGCAGAGGC
pcORF2-S+P1+P2-flag Reverse ATATCTGCAGAATTCTTAGGTCTTGAGCGAGACTGCTAGGTG