TABLE 1.
Primer name | Direction | Sequence (5′–3′) |
---|---|---|
pc | Forward | GAATTCTGCAGATATCCAGCACAGTG |
pc | Reverse | GCTCGGTACCAAGCTTAAGTTTAAACG |
pcORF2-flag | Forward | AGCTTGGTACCGAGCATGGATTACAAGGATGACGACGATAAGATGGCAGACAGGGCGGTG |
pcORF2-flag | Reverse | ATATCTGCAGAATTCTTATCACTCATGTCCGCCCTTCTC |
pcORF2-S-flag | Reverse | ATATCTGCAGAATTCTTATGGCTTTGGACCATAGTTCGAG |
pcORF2-S+P1-flag | Reverse | ATATCTGCAGAATTCTTACATTGGTGCCACTGGCAGAGGC |
pcORF2-S+P1+P2-flag | Reverse | ATATCTGCAGAATTCTTAGGTCTTGAGCGAGACTGCTAGGTG |