FIG 1.

EZH2 is crucial for the CD4 T cell response during acute viral infection. (A and B) Confocal microscopy of EZH2 in SMARTA (SM) CD4 cells from the spleens of CD45.2⁺ wild-type mice receiving adoptive transfer of CD45.1⁺ SM cells at days 2.5, 5, 8, and 30 after LCMV Armstrong infection and in naive (CD44^lo CD62L^hi) SM CD4 T cells. (C) Experimental setup. Ezh2^fl/fl SM cells (CD45.1⁺ CD45.2⁻; EZH2 WT SM) and Ezh2^fl/flCd4-Cre SM cells (CD45.1⁺ CD45.2⁺; EZH2 KO SM) were cotransferred into WT recipients (CD45.1⁻ CD45.2⁺), which were assessed on days 2.5, 8, and 55 after LCMV Armstrong infection. (D) Flow cytometry of EZH2 WT and EZH2 KO SM CD4 T cells for experiments depicted in panel C. (E) Frequency (left) and total number (right) of transferred EZH2 WT and EZH2 KO SM CD4 T cells for experiments depicted in panel C. *, P < 0.05; ****, P < 0.0001 (one-way ANOVA, Tukey’s multiple-comparison test). Data are representative of two independent experiments with at least 9 cells per group (for B, error bars are standard deviations [SDs]) or at least 4 mice per group (for D and E; error bars are SDs.).