FIG 3.

Neutralization of HPV31 lineage and sublineage variants by MAbs. (A) Consensus lineage and sublineage HPV31 L1L2 variant sequences used for creation of representative PsVs (29). (B) Neutralization sensitivity of lineage and sublineage L1 and L2 PsV variants to HPV31 MAbs. Only MAbs that had an IC80 of <0.5 μg/ml were included. Symbols indicate magnitude of difference in MAb sensitivity (IC80) between indicated variant and consensus A/A1 PsV: *, ≥4-fold reduced sensitivity. (C) HPV31 sublineage A1 homology model based upon the HPV16 L1 pentamer crystal (PDB accession number 2R5H.1) with top view shown. The quality of a predicted model was assessed by its QMEAN4 and GMQ, which were −3.35 and 0.96, respectively. All five L1 monomers of the pentamer are pictured, with external loops (gray) and all visible sites of variation from the consensus A/A1 (blue) highlighted as indicated (yellow, BC1; pink, DE2; red, EF2; orange, FG2; and green, HI1).