FIG 4.

Neutralization of HPV33 lineage and sublineage variants by MAbs. (A) Consensus lineage and sublineage HPV33 L1L2 variant sequences used for creation of representative PsVs (29). (B) Neutralization sensitivity of lineage and sublineage L1 and L2 PsV variants to HPV33 MAbs. Only MAbs that had an IC80 of <0.5 μg/ml were included. Symbols indicate magnitude of difference in MAb sensitivity (IC80) between indicated variant and consensus A/A1 PsV: *, ≥4-fold reduced sensitivity; ○, ≥10-fold reduced sensitivity; ●, ≥100-fold reduced sensitivity. (C) Graphical representation of HPV33 L1 and L2 protein combinations from A1 (white) and A2 (gray). (D) Heatmap representation of fold difference in neutralization potency (IC80) against the A1 variant. (E) HPV33 sublineage A1 L1 pentamer crystal (PDB accession number: 6IGE.2) with top view shown. All five L1 monomers of the pentamer are pictured, with external loops (gray) and all visible sites of variation from the consensus A/A1 (blue) highlighted as indicated (yellow, BC5; pink, DE4; red, EF4; orange, FG4; and green, HI5).