FIG 5.

Neutralization of HPV45 sublineage variants by MAbs. (A) Consensus sublineage HPV45 L1L2 variant sequences used for creation of representative PsVs (29). (B) Neutralization sensitivity of sublineage L1 and L2 PsV variants to HPV45 MAbs. Only MAbs that had an IC80 of <0.5 μg/ml were included. Symbols indicate magnitude of difference in MAb sensitivity (IC80) between indicated variant and consensus A/A1 PsV: +, ≥4-fold increased sensitivity; *, ≥4-fold reduced sensitivity; ○, ≥10-fold reduced sensitivity. (C) Graphical representation of HPV45 L1 and L2 protein combinations from A1 (white) and B2 (gray). (D) Heatmap representation of fold difference in neutralization potency (IC80) against the A1 variant. (E) HPV45 sublineage A1 homology model based upon the HPV18 L1 pentamer crystal (PDB accession number 2R5I.1) with top view shown. The quality of a predicted model was assessed by its QMEAN4 and GMQ, which were −2.81 and 0.99, respectively. All five L1 monomers of the pentamer are pictured, with external loops (gray) and all visible sites of variation from the consensus A/A1 (blue) highlighted as indicated (yellow, BC1; pink, DE2; red, EF2; orange, FG2; and green, HI1).