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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Osteoarthritis Cartilage. 2020 Nov 20;29(3):402–412. doi: 10.1016/j.joca.2020.11.004

Figure 2. Quantification of senescence induction within equine explants.

Figure 2.

Equine explants were divided into control and irradiated groups, cultured in explant form for 7 to 10 days, digested, and cultured in monolayer for an additional 10 to 12 days in 10% FBS for maturation of the senescence phenotype. (A) Morphology of control and irradiated chondrocytes from Equine Donor 3 after 12 days of monolayer culture; scale bar = 200 μm. (B) Representative SA-β-Gal flow cytometry plot with the region two standard deviations above control MFI marked (Equine Donor 3). (C) SA-β-Gal mean fluorescence intensity (MFI) fold increase above control quantified; (*) indicates significance by one-sample t-test compared to a hypothetical value of 1, p=0.0272. (D) SA-β-Gal flow quantified as percentage of cells SA-β-Gal high. SA-β-Gal high cells delineated as those expressing SA-β-Gal fluorescence above 2 standard deviations from the control MFI; (#) indicates significance by paired t-test, p=0.0031. (E) Information from equine donors included in the analysis (n=7 thoroughbred horses), no horses excluded from analysis.