Skip to main content
. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Prog Neurobiol. 2020 Nov 26;199:101963. doi: 10.1016/j.pneurobio.2020.101963

Fig. 4.

Fig. 4.

Nhe1 Astro-KO brains exhibited increased Wnt7a/7b expression. (A) Representative confocal images of AQP4+ and Wnt7a/7b+ positively stained vessels in wild-type and Nhe1 Astro-KO ischemic brains at 48 h Rp. Arrows: high expression of Wnt7a/7b proteins. Arrowheads: low expression of Wnt7a/7b. (B)Bar graphs show quantification of Wnt7a/7b fluorescence signal intensity. Data are ± SEM, n=6. *p < 0.05 (Student’s t-test). (C) RT-qPCR analysis of changes in expression of Wnt7a, Wnt7b, and β-catenin mRNA in the astrocytes isolated from wild-type and (A) Astro-KO brains at 24 h Rp. Data are mean ± SEM, n=4, *p < 0.05 (by Mann-Whitney Test). Representative confocal images of AQP4+ and Wnt7a/7b+ positively stained vessels in wild-type and Nhe1 Astro-KO ischemic brains at 48 h Rp. Arrows: high expression of Wnt7a/7b proteins. Arrowheads: low expression of Wnt7a/7b. (B)Bar graphs show quantification of Wnt7a/7b fluorescence signal intensity. Data are ± SEM, n=6. *p < 0.05 (Student’s t-test). (C) RT-qPCR analysis of changes in expression of Wnt7a, Wnt7b, and β-catenin mRNA in the astrocytes isolated from wild-type and Nhe1 Astro-KO brains at 24 h Rp. Data are mean ± SEM, n=4, *p < 0.05 (by Mann-Whitney Test).