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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Prog Neurobiol. 2020 Nov 26;199:101963. doi: 10.1016/j.pneurobio.2020.101963

Fig. 5.

Fig. 5.

Elevated Wnt reporter transgene expression in wild-type and Nhe1Astro-KO brainsafter ischemic stroke. (A)Breeding scheme for generation of astrocyte specific Nhe1 KO mice containing the TCF/LEF1::H2B-eGFP Wnt reporter transgene. (B) Representative confocal images showing immunofluorescence for eGFP (green) and Glut1 (cerebral vessels; red) in the IL hemispheres of Nhe1 Astro-KO and wild-type reporter mice. (C) Quantification of Wnt reporter activity (eGFP immunofluorescence) in Glut1+ vessels. Data are mean ± SEM, n=4, *p<0.05 (two-way ANOVA for followed by Tukey’s multiple comparisons test). (D) Repre-sentative images of AQP4 and β-catenin staining in the peri-lesion areas of wild-type and Nhe1 Astro-KO brains at 48 h Rp. Arrows: high expression of β-catenin protein. Arrowheads: Low expression of β-catenin. (E) Quantification of β-catenin staining intensity. Data are mean ± SEM, n = 5, *p < 0.05 (two-way ANOVA followed by Tukey’s multiple comparisons test).