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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: J Thromb Haemost. 2020 Dec 16;19(3):839–851. doi: 10.1111/jth.15184

Figure 4. DHA and its oxylipins negatively regulate platelet adhesion:

Figure 4.

DHA and its 12-LOX oxylipins, 11-HDHA and 14-HDHA, inhibit platelet adhesion and accumulation on a collagen-coated surface under arterial shear flow conditions. Sodium-citrated whole blood was treated with DMSO (Control) or increasing concentrations (5, 10 and 20μM) of A) DHA (n=6), B) 11-HDHA (n=6) or C) 14-HDHA (n=6) for 10 minutes at 37°C prior to perfusion over a collagen-coated surface at arterial shear rate (1800/s) for 4 minutes. Data represent mean ± SEM. P value denote the statistical difference between Control and treated groups. Two-way statistical ANOVA with Dunnett’s multiple comparisons post-test was performed. MFI indicates mean fluorescence intensity.