Skip to main content
. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Mol Cancer Res. 2020 Nov 10;19(3):451–464. doi: 10.1158/1541-7786.MCR-20-0453

Figure 6. Expression of the erythroid cell surface protein Glycophorin-A (GYPA) is differentially induced in KO v. WT after 5-Aza but not during terminal erythroid differentiation.

Figure 6.

A) Percentage of GYPA-positive cells over time after 5-Aza or vehicle exposure as measured by flow cytometry. Drug/vehicle was added on Days 1–3 as in previous experiments. B) Fold-change in cell-surface GYPA expression over time after 5-Aza vs. vehicle exposure. A) and B): Each point represents the mean of 2 biological replicates and 3 independent experiments, error bars represent standard error of the mean. C) Erythroid differentiation induction by recombinant erythropoietin (EPO) starting 8 days after 5-Aza or vehicle removal and ending 8 days later, measured by GYPA flow cytometry. D) Red pigmentation of cell pellets on Day 20 (Day 8 after EPO addition) is indicative of the amount of hemoglobin expression induced.