TABLE 1.
Amino acid residues of substrate binding pockets in the Val-activating A domains from OMS and other NRPS: Ohmyungsamycin synthetase A (OhmA), surfactin synthetase B (SrfAB), gramicidin S synthetase B (GrsB), and tyrocidine A synthetase C (TycC).
| A domain | Substrate specific residue (PheA numbering) [a] |
|||||||||
| 235 | 236 | 239 | 278 | 299 | 301 | 322 | 330 | 331 | 517 | |
| OhmA-A1 | D | A | Y | W | W | G | G | T | F | K |
| OhmA-A2 | D | A | Y | W | G | G | A | T | F | K |
| OhmA-A5 | D | A | Y | W | W | G | G | T | F | K |
| OhmA-A7 | D | A | Y | W | W | G | G | T | F | K |
| OhmA-A8 | D | A | Y | W | W | G | G | T | F | K |
| OhmA-A10 | D | A | Y | W | W | G | G | T | F | K |
| OhmA-A12 | D | A | Y | W | W | G | G | T | F | K |
| SrfAB-A4 | D | A | F | W | I | G | G | T | F | K |
| GrsB-A3 | D | A | F | W | I | G | G | T | F | K |
| TycC-A8 | D | A | F | W | I | G | G | T | F | K |
[a] The numbering corresponds to the residues of the PheA domain of GrsA crystal structure.