TABLE 2.

Bacterial strains and plasmids used in this study.

Strain and plasmid	Description	References
Strain
E. coli DH5α	Host for general cloning	New England Biolabs
E. coli ET12567/pUZ8002	Methylation-deficient donor strain for conjugal transfer between E. coli and Streptomyces	Kieser et al., 2000
Streptomyces sp. SNJ042	Wild-type ohmyungsamycin-producing strain	Um et al., 2013
ohmA-A2 Mut1	Gly299Trp mutation in OhmA-A2 domain of SNJ042	This work
ohmA-A2 Mut2	Ala322Gly mutation in OhmA-A2 domain of SNJ042	This work
ohmA-A2 Mut3	Gly299Trp and Ala322Gly mutation in OhmA-A2 domain of SNJ042	This work
Plasmid
pGEM T-Easy	E. coli vector for general cloning, AmpR	Promega
pKC1139	Temperature-sensitive E. coli–Streptomyces shuttle vector for gene replacement; oriT and AprR	Bierman et al., 1992
pKC-Mut1	pKC1139 derivative harboring Gly299Trp mutation in A2 domain region for gene substitution	This work
pKC-Mut2	pKC1139 derivative harboring Ala322Gly mutation in A2 domain region for gene substitution	This work
pKC-Mut3	pKC1139 derivative harboring Gly299Trp and Ala322Gly mutation in A2 domain region for gene substitution	This work