FIGURE 2.

The MIC value (μg/ml) for EntK1 (A), EntEJ97 (B), and Ampicillin (C) during blood incubation. The presented MIC values are the average of three experiments. Each bacteriocin was added to whole human blood to reach a concentration of 1 mg/ml. The samples were incubated at 37°C and then checked for antibacterial activity at time points 4, 8, 24, and 48 h using a standard MIC assay. The results indicate a 2–4-fold reduction in bacteriocin activity over the course of the experiment. The same result presented for Ampicillin, which lost fourfold activity by 48 h. RT is short for room temperature.