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. 2021 Feb 17;12:632606. doi: 10.3389/fimmu.2021.632606

Figure 1.

Figure 1

Taraxasterol (TAS) dose-dependently suppressed nigericin-induced activation of NLRP3 inflammasome in murine macrophages. Bone marrow-derived macrophages (BMDMs) (A–E) or thioglycollate (TG)-elicited peritoneal macrophages (TGPMs) (F–I) were primed with LPS (0.5 μg/ml) for 4 h and then pretreated with indicated concentrations of taraxasterol (TAS) for 1 h, followed by stimulation with nigericin (3 μM for BMDMs and 2 μM for TGPMs) for 1 h. (A,F) Western blot analysis of the expression levels of indicated proteins in the culture supernatants and cell lysates. (B,C,G,H) Histograms show the relative intensity of capase-1p10 (10 kDa) or mature IL-1β (17 kDa) bands in supernatants, with the intensity of nigericin group being set to 1.0, respectively. (D,I) Relative GSDMD_NT levels in the cell lysates. (E) The concentration of soluble IL-1β in the culture supernatants were detected by cytometric bead array (CBA) assay. Data were analyzed using the non-parametric Mann–Whitney U-test, which are shown as mean ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.