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. 2021 Feb 17;12:632606. doi: 10.3389/fimmu.2021.632606

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Copyright © 2021 Yang, Ye, Chen, Li, Wang, Zhong, Zhong, Zeng, Xu, He and Ouyang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TAS inhibited mTOR signaling in LPS-primed macrophages. LPS-primed BMDMs were primed with 0.5 μg/ml LPS for 4h, followed by treatment with 100 μM TAS for indicated time lengths (A), or indicated concentration of TAS for 15 min (B), or 100 μM TAS, 20 nM rapamycin (RAPA), 30 nM INK-128 (INK), respectively (C). The levels of indicated proteins in the cell lysates were analyzed by Western blotting. Actin was added as loading control. Data are shown as mean ± SD (n = 5). **P < 0.01; ***P < 0.001; ns, not significant.