Fig 6. Adoptive transfer of Notch-primed DCs limited inflammation associated transformation.
(A) Schematic of in vitro priming of bone marrow-derived DCs with OP9-DLL1 in the presence of Flt3L and adoptive transfer. (B) Colon inflammation and dysplastic scores were recorded 5 days after the last dose of DC transfer (n=9) or PBS injection (control; n=6). Data were pooled from 3 experiments. (C) Representative H&E staining of colonic tissues from Fx−/− mice received PBS (control) or primed DCs. (D) Inflammatory cytokine mRNA expression in colon tissue from Fx−/− mice receiving PBS or DC injection was measured by qRT-PCR and normalized to the expression in Fx−/− mice receiving PBS. Results shown in B and D are mean ± SD. Student t-test was performed; *p<0.05, **p<0.01