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. 2021 Mar 2;7:44. doi: 10.1038/s41420-021-00425-z

Fig. 3. XBP1 downregulation protects TCMK-1 cells against H/R via regulation of HRD1, NRF2, and HO-1.

Fig. 3

A The level of ROS was attenuated by lenti-shRNA-Xbp1 transduction in H/R-exposed TCMK-1 cells. DCFH-DA assay; **P < 0.01 vs. lenti-control H/R group at each time point (n = 6). B P53, ATM, and cleavage caspase 3 expression were ascended by lenti-Xbp1 transduction and descended by lenti-shRNA-Xbp1 transduction in H/R-exposed TCMK-1 cells. Western blot; **P < 0.01 vs. lenti-control H/R group (n = 6). C The apoptotic proportion of H/R-exposed TCMK-1 cells was decreased by lenti-shRNA-Xbp1 transduction, as revealed by flow cytometry. **P < 0.01 vs. lenti-control H/R group at each time point (n = 6). D The BrdU incorporation (proliferative capability) of H/R-exposed TCMK-1 was enhanced by lenti-shRNA-Xbp1 transduction. *P < 0.05 vs. lenti-control H/R group at each time point (n = 6). E HO-1 expression was negatively modulated by XBP1 expression following lenti-Xbp1 and lenti-shRNA-Xbp1 transduction. Western blot; **P < 0.01 vs. lenti-control H/R group (n = 6). F XBP1 positively and negatively regulated HRD1 and NRF2, respectively. *P < 0.05, **P < 0.01 vs. lenti-control H/R group (n = 6).