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. 2021 Mar 2;11:4983. doi: 10.1038/s41598-021-82353-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of presynaptic input from interbrain (IB) and hippocampal formation (HPF) to VISp. (a) Top, horizontal and sagittal projections, showing the distribution of labelled rabies-infected cells within the IB. Dashed lines indicate location of cross-section images, below. Bottom, coronal cross-sections of the template brain at three locations, with the position of labelled cells in the interbrain. Brain regions are outlined, and cells ± 20 μm anterior–posterior of the cross-section location are shown. Lateral preoptic area (LPO), lateral dorsal nucleus (LD), reticular nucleus (RT), lateral posterior nucleus (LP), and dorsal (LGd) parts of the lateral geniculate complex are indicated (in brackets, the parent regions of these regions). Numbers below indicate the number of cells from each line appearing in the image. (b) Sagittal projection showing the distribution of labelled cells within the HPF. Dashed lines indicate location of cross-section images, below. Below, coronal cross-sections of the template brain at three locations, with the position of labelled HPF cells and brain regions outlined (includes cells ± 10 μm anterior–posterior of the cross-section location). Pre- (PRE), post- (POST) and para- (PARA) subiculum, as well as lateral (ENTl) and medial (ENTm) entorhinal cortex are indicated. The dark grey region indicates a region labelled HPF with no further subdivision (NOS not otherwise specified). (c) Proportion of rabies-infected cells within the subregions of the interbrain (IB) and cortical plate (CTXpl). Error bars show the S.E.M. and data from individual mice are shown as dots overlaying the bars.