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. 2021 Mar 2;11:4997. doi: 10.1038/s41598-021-84660-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Scanning electron micrographs (A–D) to visualize the microvilli density and goblet cells containing neutral mucins (E–H, PAS stain, 40 × magnification) in the distal intestine (E–H) and rectum (I–L) of barramundi fed Control (Diet1), 80PBM_10TH+10HI (Diet2), 85PBM_5TH+10HI (Diet3), and 90PBM_5TH+5HI (Diet4) for six weeks. Variation in the microvilli density (M) and neutral mucins in the distal intestine (N) and rectum (O) of barramundi fed test diets. Multiple comparisons were performed by one-way ANOVA analysis followed by Dunnett’s multiple comparisons test. NM, neutral mucins and ns, not significant.