Table 1.
Ranking of the candidate reference genes according to their stability value calculated by NormFinder.
| Rank | Somatic embryogenesis group | Germination group | ||
|---|---|---|---|---|
| Gene name | Stability value | Gene name | Stability value | |
| 1 | EF1g | 0.0024 | EF1g | 0.0013 |
| 2 | HIS1 | 0.0046 | ACT | 0.0016 |
| 3 | GAPDH | 0.0055 | Rpl2 | 0.0030 |
| 4 | TUA | 0.0063 | HIS1 | 0.0035 |
| 5 | Rpl2 | 0.0088 | EF1a | 0.0062 |
| 6 | ACT | 0.0092 | GAPDH | 0.0066 |
| 7 | UBQ | 0.0102 | UBQ | 0.0075 |
| 8 | EF1a | 0.0119 | TUB | 0.0097 |
| 9 | TUB | 0.0126 | TUA | 0.0114 |
| 10 | 18S rRNA | 0.0210 | 18S rRNA | 0.0190 |
The input data for NormFinder are supposed to be on a linear scale. The raw Ct values were transformed to linear scale expression quantities using the standard curve or delta-Ct method.