Table 3.
Expression stability of reference genes analyzed by geNorm, NormFinder, BestKeeper and delta Ct.
| Stability rank | Somatic embryogenesis group | Germination group | ||||||
|---|---|---|---|---|---|---|---|---|
| geNorm | NormFinder | BestKeeper | ΔCT | geNorm | NormFinder | BestKeeper | ΔCT | |
| 1 | EF1g | EF1g | EF1g | EF1g | EF1g | EF1g | EF1g | EF1g |
| 2 | TUA | HIS1 | HIS1 | HIS1 | ACT | ACT | GAPDH | ACT |
| 3 | GAPDH | GAPDH | TUA | TUA | HIS1 | HIS1 | HIS1 | GAPDH |
| 4 | HIS1 | TUA | GAPDH | GAPDH | GAPDH | Rpl2 | ACT | HIS1 |
| 5 | ACT | Rpl2 | EF1a | EF1a | UBQ | EF1a | EF1a | Rpl2 |
| 6 | EF1a | ACT | Rpl2 | ACT | Rpl2 | GAPDH | UBQ | UBQ |
| 7 | UBQ | UBQ | ACT | UBQ | EF1a | UBQ | TUA | EF1a |
| 8 | TUB | EF1a | TUB | Rpl2 | TUA | TUB | Rpl2 | TUB |
| 9 | Rpl2 | TUB | UBQ | TUB | TUB | TUA | TUB | TUA |
| 10 | 18S rRNA | 18S rRNA | 18S rRNA | 18S rRNA | 18S rRNA | 18S rRNA | 18S rRNA | 18S rRNA |