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. 2021 Mar 2;11:4970. doi: 10.1038/s41598-021-84512-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Adipocyte differentiation of Plap-1 knock-down 3T3-L1 cells by siRNA. (A) Real-time PCR analysis was performed to confirm Plap-1 knock-down by siRNA in 3T3-L1 cells. Data are represented as relative expression to control siRNA. Values represent the mean ± SD triplicate assays (n = 3). (B) Oil Red O staining of Plap-1 knock-down and control 3T3-L1 cells was conducted after adipocyte differentiation. (C) Quantitative lipid assay of Plap-1 knock-down and control 3T3-L1 cells was performed after Oil Red O staining (n = 4 in each group). Values represent the mean ± SD (n = 4). (D) Real-time PCR analysis was performed to examine adipogenic gene expressions of Plap-1 knock-down 3T3-L1 cells during adipocyte differentiation. Results show the mean ± SD of triplicate assays (n = 3). *: p < 0.05, **: p < 0.01.