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. 2021 Mar 2;11:4933. doi: 10.1038/s41598-021-84303-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of amino acid receptors mRNAs along the rainbow trout GIT. For (a–e), quantitative analysis of mRNA abundance was performed by RT-qPCR considering actb and ee1af1α as reference genes. Data are expressed as mean + SEM (n = 6), relative to the tissue with the lowest mRNA abundance. For all genes, one sample from each tissue was run on a 1.5% agarose gel and representative bands are shown; please refer to Supplementary Fig. S1 for entire gels. Ant. middle int., anterior middle intestine; casr, calcium-sensing receptor; gprc6a, Dist. int., distal intestine; G protein-coupled receptor family C group 6 member A; grm4, metabotropic glutamate receptor 4; Interm. middle int., intermediate middle intestine; Post. middle int., posterior middle intestine; Prox. int., proximal intestine; tas1r1, tas1r2a, tas1r2b, tas1r3, taste receptor 1 family member 1, 2a, 2b, and 3.