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. 2021 Mar 2;11:4951. doi: 10.1038/s41598-021-84329-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Bulk characterization of encapsulin cargo loading. (A) Experimental plan outlining the production and encapsulation of heterologous fluorescent cargo to test targeting peptide strength. (B) and (C) SDS-PAGE gels and negatively stained transmission electron micrographs of purified encapsulin particles using native TPs. (D) and (E) Bulk fluorescence measured for the indicated systems and targeting peptides. Error bars represent standard deviations resulting from three independent replicates.