Figure 5.

Design and characterization of novel targeting peptide mutants. (A) Workflow outlining the design and testing of mutant targeting peptides. (B) Heat map of computationally generated point mutations within the M. xanthus T3 targeting peptide. The color gradient represents the Rosetta Energy score (blue: improved binding, red: worse binding). Native sequence residues are indicated by asterisks. The mutants selected for experimental testing are shown with black arrows pointing from the native residues (asterisk) to the mutant residues. (C) Bulk fluorescence measurements of mutant targeting peptides. Error bars represent standard deviations resulting from three independent replicates. Fluorescence measurements of the native and control TP for the T3 system are shown for comparison.