Fig. 2. Vaccine-specific IFN-γ⁺ T cells and IgG antibodies exhibit cross-reactivity to GP from heterologous LASV strains.

a CD-1 mice were vaccinated with ChAdOx1-Lassa-GPC (n = 8) or ChAdOx1-GFP (n = 2) and euthanized after 14 days for the collection of splenocytes. IFN-γ-secreting T cells were quantified by ELISpot assay after stimulation with four different peptide pools corresponding to the full-length GPC sequence from a representative strain from LASV lineages I–IV. Responses are reported as spot forming units (SFU) per 1.0 × 10⁶ splenocytes. The dotted line indicates the mean response in ChAdOx1-GFP vaccinates. b CD-1 mice were vaccinated with ChAdOx1-Lassa-GPC (n = 8) or ChAdOx1-GFP (n = 2) and euthanized after 28 days for sera collection. IgG antibody titers against GP from representative strains from LASV lineages II–IV were measured in twofold serial-diluted sera by ELISA. Josiah strain GP (lineage IV) is homologous to the vaccine antigen. The magnitudes of IFN-γ⁺ T-cell responses and titers of IgG antibodies displaying reactivity to each heterologous strain were determined to be statistically comparable to Josiah strain-specific responses by one-way ANOVA with Dunnett’s multiple comparisons test.