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. 2021 Mar 2;5:14. doi: 10.1038/s41698-021-00153-8

Fig. 6. CYLD inhibits EBV reactivation dependent on p18.

Fig. 6

a Representative IHC staining of CYLD and EAD expression taken from pathological sections of NPC patients (100×: scale bar, 100 μm; 400×: scale bar, 50 μm). b EAD expression level was calculated based on CYLD expression. High and low groups were according to median expression. c, d HK1-EBV and HONE1-EBV cells infected with vector or CYLD and total RNA from cells was isolated and subjected to real-time PCR. e, f Real-time PCR showing EBV DNA copy levels of EBV in HK1-EBV and HONE1-EBV cells. HONE1-EBV cells were infected with p18 lentiviral shRNAs and then transfected with the indicated constructs. g Cell lysates were then extracted and subjected to Western blotting. h Total RNA and i gDNA were isolated and subjected to real-time PCR. j Cell lysates were immunoblotted with antibodies against the indicated proteins. β-Actin was used as a control to confirm equal loading of protein. (Data are represented as mean ± SEM (n = 3). Differences were considered significant at *p < 0.05, **p < 0.01, ***p < 0.001).