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. 2021 Mar 2;11:4952. doi: 10.1038/s41598-021-83938-y

Figure 3.

Figure 3

Curcumin induces the expression of PGC1-α and COX1. The expression of PGC1-α and COX1 was evaluated in OPs treated for 24 h with 1 μM curcumin (curc). To verify whether the effects of curcumin on PGC1-α and COX1 expression were PPAR-γ- and/or ERK-dependent, OPs were pretreated for 30 min with 1 μM GW9662 (GW; PPAR-γ antagonist) or 10 μM PD98059 (PD; ERK1/2 inhibitor). Representative photomicrographs of PCG1-α (panel A; green) or COX1 (panel B; green) are shown. Nuclei were stained using the Hoechst 33258 nuclear fluorochrome (blue, H). Mean fluorescence intensities (MFI value) are shown in the lower respective panels. Data are means ± SEM of 200–250 cells for condition. Western blot of PCG1-α and COX1 expression are shown in C and D, respectively. Representative western blot from same gel of PCG1-α or COX1 and β-actin (used as an internal control) are shown. Data are means ± SEM of three independent experiments (*p < 0.001 vs. CTR; **p < 0.01 vs .curc). Scale bar 10 µm. Image Lab 4.0 software (Bio-Rad, http://www.bio-rad.com/en-us/sku/1709690-image-lab-software) and Leica Application Suite Software (260RI, https: //www.leica-microsystems.com/products/microscope-software/details/product/leica-las-x-ls/) were used respectively for Wb and IF image acquisitions.