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. 2021 Mar 2;11:4952. doi: 10.1038/s41598-021-83938-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Curcumin protects from the effect of TNF-α on PGC1-α and COX1 expression. PGC1-α and COX1 expression levels were evaluated in cells treated for 24 h with curcumin (curc) and/or TNF-α. Representative photomicrographs are shown (A). Nuclei were stained using the Hoechst 33258 nuclear fluorochrome (blue, H). Representative western blot of PCG1-α or COX1 and β-actin (used as an internal control) are shown (B). The intensities of bands corresponding to PCG1-α or COX1 and β-actin were measured by densitometric analysis and the ratio PCG1-α/β-actin or COX1/β-actin is given as percentage on untreated cells. Data are means ± SEM of three independent experiments *p < 0.05 versus CTR, **p < 0.005 versus curc + TNF-α. Scale bar 10 µm. Image Lab 4.0 software (Bio-Rad, http://www.bio-rad.com/en-us/sku/1709690-image-lab-software) and Leica Application Suite Software (260RI, https: //www.leica-microsystems.com/products/microscope-software/details/product/leica-las-x-ls/) were used respectively for Wb and IF image acquisitions.