Fig. 7. Arg-II deficiency reduces age-associated TGF-β1 in female mice.

a mRNA levels of tgf-β1 in young and old WT and arg-II^−/− mouse kidneys analyzed by qRT-PCR; n = 8 in each group; b Central transverse renal sections (one section from each animal) were prepared from young and old WT and arg-II^−/− female mice and subjected to immunofluorescence staining of ACE-1 (red) and TGF-β1 (green). Representative images were selected from the outer medulla. n = 6 in each group. Scale bars = 0.1 mm. c Quantification of the intensity of fluorescence signals of TGF-β1. Y-WT young WT, Y-KO young arg-II^−/−, O-WT old WT, O-KO old arg-II^−/−. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.