Fig. 8. Silencing arg-II decreases TNF-α induced expression of adhesion molecules in HK-2 cells.
a HK-2 cells were serum starved for 24 h, then incubated in the presence of various concentration or various duration of TNF-α. Cell lysates were prepared and subjected to immunoblotting analysis of VCAM-1, ICAM-1, Arg-II, and β-actin. β-actin served as loading control. b HK2 cells were transduced with rAd/U6-LacZshRNA as control (LacZ) or rAd/U6-Arg-IIshRNA to silence arg-II gene (arg-II). 2 days post-transduction, cells were serum starved for 24 h, then incubated with 10 ng/ml TNF-α for 24 h. Cell lysates were prepared and subjected to immunoblotting analysis of VCAM-1, ICAM-1, Arg-II, and β-actin. Data are presented as mean ± SEM. n = 4. *P ≤ 0.05, ***P ≤ 0.001. rAd recombinant adenovirus.