Fig. 6.

Determination of reactive oxygen species generation via detection of DCFDA by flow cytometry. THP-1 cells were incubated with high glucose for different time points and stained with DCFDA. The fluorescence intensity was measured by flow cytometry. A shift to the right indicates increased reactive oxygen species levels (a). The mean fluorescence intensity (MIF) values are presented as means ± SD of three experiments (b). Statistical analysis was performed using by the Kruskal-Wallis test. *p < 0.05