Fig. 10.

HPLC chromatograms showing the same retention time (5.85 min, shown with arrow) for the ST, ST-S, and the MUS1 spot obtained in the TLC assay. The crude extract was extracted from fermented broth using Potato dextrose broth (PDB) as a culture medium using ethyl acetate (EA). The EA-extracted crude extract was run on TLC plates against standard Taxol (ST—directly prepared by dissolving standard Taxol powder in methanol). Both spots ST-S (an ST co-run with MUS1, scrapped from TLC and membrane filtered later) and MUS1 were scrapped from the silica, membrane filtered, and analyzed using reverse-phase HPLC (RP-HPLC). ST and ST-S are the authentic Taxol standard while MUS1 corresponds to the putative Taxol compound obtained from the EA fraction of isolate MUS1 (also scrapped from TLC and membrane filtered). A similar chromatogram before and after the Taxol peak in MUS1 and ST-S represents the fingerprints of scrapped silica