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TLC-bioautography assay against Pseudomonas aeruginosa ATCC 27853
| Spot number | Rf value | ZOI |
|---|---|---|
| 1 | 0.25 | |
| 2 | 0.34 | |
| 3 | 0.4 | |
| 4 | 0.45 | |
| 5 | 0.51 | |
| 6 | 0.55 | |
| 7 | 0.6 | |
| 8 | 0.82a | Positive |
| 9 | 0.98 |
a Inhibitory activity, after TLC separation of the ethyl acetate (EA) fraction from isolate MUS1, was attributed to one major constituent, spot 8 (Rf = 0.82), which gave a clear zone of inhibition (ZOI) seen against a purple background
Rf retardation factor
